Part:BBa_K731711:Design

BBa_K731710 backbone with T7terminator (BBa_K731721)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 6865
  Illegal suffix found in sequence at 1
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6865
  Illegal NheI site found at 6894
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 6871
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6865
  Illegal BglII site found at 6011
  Illegal BamHI site found at 6847
  Illegal XhoI site found at 753
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 6865
  Illegal suffix found in sequence at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 6865
  Illegal XbaI site found at 6880
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 714
  Illegal NgoMIV site found at 1051
  Illegal NgoMIV site found at 4231
  Illegal NgoMIV site found at 4391
  Illegal NgoMIV site found at 5979
  Illegal AgeI site found at 6815
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2228
  Illegal SapI.rc site found at 3310
  

Design Notes

The measurements with this plasmid were made using the E. coli strain BL21(DE3)pLysS. This is a lysogen strain that expresses T7 RNA polymerase, and for this reason allows comparison between different RNA polymerases. The expression of this plasmid in this strain give a considerable basal expression. This feature can be used to sceen the colonies in the ligation plate.

Source

This is a composite part made of BBa_K731721 inserted in the BBa_K731710 backbone. The backbone is derived from the RL024A, a plasmid made by Roberta Lentini and kindly provided to the Trento iGEM team by the Mansy lab. The terminator has been extracted by PCR from pET21b vector.